Nucleotide sequence of the cDNA of a bovine 70 kilodalton heat shock cognate protein.

نویسندگان

  • C DeLuca-Flaherty
  • D B McKay
چکیده

The 70 kD heat shock cognate proteins (HSC70s) are constitutive members of the 70 kD heat shock related protein family. We have sequenced the cDNA of a bovine HSC70: clones were isolated from a bovine brain cDNA library in XgtlO (1) by hybridization with probes derived from a rat HSC70 cDNA clone (2); one full-length clone was sequenced. The deduced amino acid sequence matches CNBr-generated peptide sequence of purified bovine brain hsc70 and is strikingly similar to that of rat HSC70 (2), differing at only 3 positions. ACKNOWLEDGEMENTS

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Structural organization of the spinach endoplasmic reticulum-luminal 70-kilodalton heat-shock cognate gene and expression of 70-kilodalton heat-shock genes during cold acclimation.

The 70-kD heat-shock proteins (HSP70s) are encoded by a multigene family in eukaryotes. In plants, the 70-kD heat-shock cognate (HSC70) proteins are located in organellar and cytosolic compartments of cells in most tissues. Previous work has indicated that HSC70 proteins of spinach (Spinacia oleracea) are actively synthesized during cold-acclimating conditions. We have isolated, sequenced, and ...

متن کامل

The production of molecular clones of Toxoplasma gondii-derived heat shock protein 70 kDa

Toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. Toxoplasma gondii-derived heat shock protein 70 (Hsp70) may play an important role in the virulence of Toxoplasma gondii (T. gondii). In the present study, T. gondii Hsp70 was amplified by polymerase chain reaction (PCR) from the DNA of the T. gondiitachyzoite RH strain through the use o...

متن کامل

Chemical modifications of a recombinant bovine stress-inducible 70 kDa heat-shock protein (Hsp70) mimics Hsp70 isoforms from tissues.

A cDNA clone for the stress-inducible 70 kDa heat-shock protein (Hsp70) has been isolated from a bovine skeletal-muscle cDNA library. This mRNA encodes a protein with a calculated molecular mass of 70250 Da. The cDNA has one continuous open reading frame capable of encoding a 641-amino-acid protein. Expression of this cDNA in a bacterial expression system produced a protein with a mobility iden...

متن کامل

Expression of Recombinant Heat-Shock Protein 70 of MCAN/IR/96/LON-49, a Tool for Diagnosis and Future Vaccine Research

Background: Heat shock protein 70 (HSP70) is present in all organisms studied so far, and is a major immunogen in infections caused by pathogens including Leishmania spp. Objective: The aim of this study was to clone and express HSP70 from L. infantum strain MCAN/IR/96/LON-49 and evaluate antibody response against HSP70 in visceral leishmaniasis (VL). Methods: The L. infantum HSP70 gene segment...

متن کامل

LEVELS OF ANTI-STREP TOKINASE AND ANTI-MYCOBACTERIAL HEAT SHOCK PROTEIN 65 KILODALTON (ANTI-MHSP 65) ANTIBODIES IN PATIENTS WITH AUTOIMMUNE DISEASES

Bacterial and viral triggers are suspected agents in the initial etiology of autoimmune diseases. There are some studies on the etiology of autoimmune disorders which have focused on streptococcal infection and a possible relation with microbial heat shock proteins (hsp) which show significant homology with human heat shock proteins. In addition, some serotypes of streptococci cross-react ...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Nucleic acids research

دوره 18 18  شماره 

صفحات  -

تاریخ انتشار 1990